Yusuke's recovery speed is extremely quick due to genkai's spirit wave to the point that he can recover from exhaustion in a matter of minutes. i don't really think that it matters because yusuke would only need to expel a minimal amount of energy to run through #1-4 on this list and while Hiei was strong at the end of the series he was no match for an upper level S class demon Like yusuke (or in his case mukuro)
@hitsusatsu11: It was pretty clear that Hiei wasn't in Mukuro's league. Throughout their battle she played with him, she simply dodged/blocked his attacks without counterattacking herself. Hiei at best was a Mid tier S, no Match for the kings. Like i said earlier i don't believe yusuke will have to use much energy to beat #1-4 on the gauntlet and unless yusuke is drained of quite a bit of power, Hiei won't be able to beat him.